General Cell Lines and Cell Cultures
ATCC L-WRN; Transfected Fibroblast; Mouse (Mus musculus)
For-profit Organizations: For every order of this item, you must work directly with the contributor, Washington University in St. Louis to (i) negotiate a research-use license, and/or (ii) have the contributor provide authorization to ATCC to ship this item under your existing license. We cannot ship this item until we receive communication directly from Washington University in St. Louis that we are authorized to ship each order. We are providing the following contact information, but this information may change without notice: Washington University, Office of Technology Management, Attn. Associate Director, Phone: 314-747-0920 Email: otm@dom.wustl.edu. Once ATCC has received authorization from Washington University in St. Louis, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team.
ATCC Mammary Epithelial Cell Basal Medium
Mammary Epithelial Cell Basal Medium is a sterile, phenol-red-free, liquid tissue culture medium designed to support epithelial cells derived from a normal patient's breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040). ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three-dimensional culture, and carcinogen screening.
Eurofins Discoverx cAMP Hunter CHO-K1 RXFP1 Gs Cell Line, 2 vials. (95-0127C2)
The cAMP Hunter RXFP1 (LGR7) Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. relaxin-2) based activation of RXFP1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type RXFP1, a naturally Gs coupled GPCR. Upon activation of RXFP1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
System Biosciences LLC 293TN PRODUCER CELL LINE293TN Producer Cell Line = 2 x 10^6 Cells
Delivering high titer lentivirus prepsOptimized for effective, high-titer production of pseudoviral particles, SBI’s 293TN Producer Cell Line has been transformed with the SV40 large T antigen to promote robust growth and features the neomycin resistance marker for stable propagation. The 293TN packaging cell line is shipped at low passage (P2).
Miltenyi Biotec, Inc. CD4/CD8 (TIL) MicroBeads, mouse
CD4/CD8 (TIL) MicroBeads, mouse have been optimized for the isolation of CD4+ and CD8+ tumor-infiltrating lymphocytes from mouse tumors
Biochain Institute Inc Paraffin Tissue Section - Parkinson's Disease: Brain: Medulla oblongata, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from single human diseased sample. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx PathHunter GRPR Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0667C3)
The PathHunter GRPR Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of GRPR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GRPR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GRPR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Stomach, 0.2 x2 mg /PK
BioChain's human total protein matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor andcorresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designedfor identifying tumor-specific genes and tumor metastasis genes.
Eurofins Discoverx PathHunter CHO-K1 TACR1 β-Arrestin Cell Line, 2 vials. (93-0344C2)
The PathHunter TACR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. substance P) based activation of TACR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TACR1 and EA-tagged β-Arrestin-2. Activation of TACR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mNPY2R β-Arrestin Cell Line, 2 vials. (93-0569C2)
The PathHunter mNPY2R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. peptide YY) based activation of mNPY2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mNPY2R and EA-tagged β-Arrestin-2. Activation of mNPY2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen Motilin Receptor Stable Cell Line, 2 vials. (HTS121C)
The ChemiScreen Motilin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MLNR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MLNR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen α1B Adrenergic Receptor Membrane Preparation, 1 vial. (HTS158M)
The ChemiScreen α1B Adrenergic Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRA1B surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Electron Microscopy Sciences Quadruple Geological Thin Section Holder, 8.5 mm Pin
This thin section holder made from aluminum with copper clips and brass screws. The unit measures 107x58x8 mm and it holds four standard geological thin sections or slides of 47 x 27 mm. The holder has convenient tweezer inserts which allow for easy loading. Clips are provided for secure holding.
Type: 8.5 mm Pin